![]() ![]() Thermostability analysis indicates that these LPMOs can refold spontaneously following denaturation dependent on the presence of copper coordination. In addition, CflaLPMO10B, CflaLPMO10C, and CfiLPMO10 exhibit limited capacity to perform mixed C1/C4 regioselective oxidative cleavage. We demonstrate that these four enzymes oxidize insoluble cellulose with C1 regioselectivity and show a preference for substrates with high surface area. Here, we present the comprehensive biochemical characterization of three AA10 LPMOs from Cellulomonas flavigena ( CflaLPMO10A, CflaLPMO10B, and CflaLPMO10C) and one LPMO from Cellulomonas fimi ( CfiLPMO10). The Creative Commons Public Domain Dedication waiver ( ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. This work also contributes to the broader mapping of enzyme activity in Auxiliary Activity Family 10 and provides new biocatalysts for potential applications in biomass modification. Further, we demonstrate that the LPMOs encoded by Cellulomonas flavigena exhibit synergy in cellulose degradation, which is due in part to decreased autoinactivation.ConclusionsTogether, these results advance understanding of the cellulose utilization machinery of historically important Cellulomonas species beyond hydrolytic enzymes to include lytic cleavage. ![]() Scanning and transmission electron microscopy revealed substrate-specific surface and structural morphological changes following LPMO action on Avicel and phosphoric acid-swollen cellulose (PASC). ![]() Cellulomonas bacteria have a rich history of study due to their ability to degrade recalcitrant cellulose, yet little is known about the predicted LPMOs that they encode from Auxiliary Activity Family 10 (AA10).ResultsHere, we present the comprehensive biochemical characterization of three AA10 LPMOs from Cellulomonas flavigena (CflaLPMO10A, CflaLPMO10B, and CflaLPMO10C) and one LPMO from Cellulomonas fimi (CfiLPMO10). Schiller of the United States District Court for the Eastern District of Pennsylvania.BackgroundThe discovery of lytic polysaccharide monooxygenases (LPMOs) has fundamentally changed our understanding of microbial lignocellulose degradation. in Religious Studies from Georgetown University. from New York University School of Law, and his M.A.L.S. in Religious Studies from the University of Pennsylvania, his J.D. Noti was a litigation associate at Paul, Weiss, Rifkind, Wharton & Garrison. He returned to the Commission in 2012 as Acting Assistant General Counsel in the Litigation Division and in 2013 he was named Acting Associate General Counsel for Policy. Noti was appointed Special Assistant United States Attorney in the United States Attorney’s Office for the District of Columbia. Noti joined the Commission in 2007 in its Litigation Division, where he served as an attorney from 2007 to 2011. Noti will oversee the FEC’s Policy Division, which counsels the Commission regarding advisory opinions, rulemakings, audits, and other matters requiring interpretation of federal campaign finance law. WASHINGTON - The Federal Election Commission has appointed Adav Noti as Associate General Counsel. ![]()
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